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Discovered in 1982, the bacterium Borrelia burgdorferi is the aetiologic agent
of Lyme borreliosis, a disease which is transmitted by different tick species of
the genus Ixodes. Lyme borreliosis is a multisystemic disorder that can affect
several organs, such as skin, nervous system, large joints and cardiovascular
system. Even though Lyme disease spirochaetes elicit a vigorous immune response,
Borrelia bacteria survive and persist in the circulation of infected patients.
Similarly to syphilis, Lyme borreliosis generally progresses through different
clinical stages, from early to late infection: |
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– Stage 1: skin lesion at the site of tick bite; early infection with localized
exanthema (erythema chronicum migrans, EM) may be followed by disseminated
infection if untreated. |
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– Stage 2: neurological disorders (neuroborreliosis). |
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– Stage 3: arthritis that can be observed even years after infection. |
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Similarity of clinical symptoms between Lyme borreliosis and unrelated diseases
poses diagnostic concerns due to variety of highly variable manifestations.
Diagnosis of borreliosis may be difficult on the basis of clinical findings
especially in the absence of an anamnesis of tick bite or of erythema chronicum
migrans. In addition, the disease may remain asymptomatic until later stages.
Antibody detection is used to determine the cause of illness. The use of
whole-cell sonicated Borrelia burgdorferi can yield well-documented
false-positive results, due to cross-reactivity of specific antibodies with
proteins having high homology with various bacterial pathogens, especially
Treponema pallidum, the etiologic agent of syphilis. Diagnostic tests using
bacterial lysate such as antigen, even from different strains of Borrelia
burgdorferi, frequently fail to achieve conclusive results in the early stage of
infection. |
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Detection of Borrelia burgdorferi specific antibodies in cerebrospinal fluid is
more significant than in serum or plasma and is considered a proof of
intrathecally-synthesized antibodies (i.e., neuroborreliosis) when evaluated in
association with other laboratory findings. |
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DiaSorin Liaison Borrelia IgG |
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- 2-step immunoluminometric sandwich assay using directly coated magnetic
microparticles
- Sample matrix: Serum, Plasma, CSF
- Sample volume: 5 µl serum, 50µl CSF
- Incubation time: 20 min
- Measuring range: 0 – 240 AU/ml
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DiaSorin Liaison Borrelia IgM II (qualitative assay) |
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- 2-step immunoluminometric sandwich assay using directly coated magnetic
microparticles
- Sample matrix: Serum, Plasma
- Sample volume: 10 µl serum
- Incubation time: 20 min
- Measuring range: 0 – 6 index
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DiaSorin Liaison Borrelia IgM Quant |
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- 2-step immunoluminometric sandwich assay using directly coated magnetic
microparticles
- Sample matrix: Serum, Plasma, CSF
- Sample volume: 10 µl serum, 50µl CSF
- Incubation time: 20 min
- Measuring range: 0 – 190 AU/ml
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Product code |
Product name |
Method |
Kit Size |
Sample Type |
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310880 |
Liaison Borrelia IgG |
2-step immunoluminometric sandwich assay |
100 test kit |
Serum, Plasma, CSF |
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310010 |
Liaison Borrelia IgM II |
2-step immunoluminometric sandwich assay |
100 test kit |
Serum, Plasma |
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310020 |
Liaison Borrelia IgM Quant |
2-step immunoluminometric sandwich assay |
100 test kit |
Serum, Plasma, CSF |
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BORG0040 |
Borrelia burgdorferi IgG |
ELISA |
96 wells |
Serum |
| BORM0040 |
Borrelia burgdorferi IgM |
ELISA |
96 wells |
Serum |
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